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KMID : 0525720160210020075
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Journal of Chitin and Chitosan
2016 Volume.21 No. 2 p.75 ~ p.81
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Sargassum fusiforme Fucoidan: Desalination, Components, Antioxidant, and NO Inhibition in LPS-Stimulated RAW 264.7 Macrophages
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Park Hyeon-Ho
Jung Won-Kyo
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Abstract
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Sargassum fusiforme fucoidan (SFF) was desalinated using electrodialysis system. Desalination of SFF was successfully confirmed by conductivity and content of sodium chloride (NaCl). The contents of polyphenol and carbohydrate in the desalinated (DSFF) and non-desalinated SFF (NDSFF) were also determined. After determination of DSFF and NDSFF contents, both samples were subjected to evaluate antioxidant activity and anti-inflammatory inhibitory activity of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The DSFF significantly increased (p<0.05) the polyphenol (4.04%) and carbohydrate (48.96%) contents compared to NDSFF (3.61% and 38.73%). Additionally, DSFF significantly enhanced (p<0.05) scavenging activities against 2,2-diphenyl-1-pricylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzthiazoline)-6- sulphonic acid (ABTS) and hydrogen peroxide (H2O2) compared to NDSFF. In inhibitory activity of NO production, DSFF and NDSFF significantly inhibited (p<0.05) NO production, but no significant differences between the DSFF and NDSFF in LPSstimulated RAW 264.7 macrophages. These data indicate that DSFF has higher antioxidant activity than NDSFF. In conclusion, the application of electrodialysis system could be useful in standardization of both bioactivity and content for marine bioactive substances with a large amount of salt.
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KEYWORD
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Sargassum fusiforme fucoidan extract, Desalination, Antioxidant activities, Anti-inflammatory activity
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